Joshi, Dr. Gnanada

Title of Research Project:

Effects of low doses of radiation on Drosophila melanogaster

Research Description:

Ionizing radiation causes either direct breakage of one or both strands of DNA or it can cause indirect damage by free radical formation. The dose-effect relationships of cells exposed to ionizing radiation are frequently described by linear quadratic (LQ) models over an extended range of doses. My previous studies demonstrated that cytogenetic effects of low dose exposures are cell cycle-dependent and are underestimated, hence do not provide accurate risk assessments. I propose to evaluate mechanisms of DNA damage at low doses in-vivo in Drosophila melanogaster and also elucidate its effects in human radiation sensitive and resistant cells. We will utilize the power of Drosophila genetics to evaluate mitotic checkpoint defects at low-dose exposures. We will also perform cytogenetic evaluations of low dose effects on human cancer cells.

If there is important prerequisite knowledge/skills required for students, please describe below:

Knowledge of Genetics is preferred